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KMID : 0364820110470040289
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Korean Journal of Microbiology
2011 Volume.47 No. 4 p.289 ~ p.294
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Cold Shock Response and Low Temperature Stable Transcript of DEAD-box RNA Helicase in Bacillus subtilis
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Oh Eun-Ha
Lee Sang-Soo
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Abstract
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We investigated the cold shock sensitivity of DEAD-box RNA helicase gene deleted strains of in Bacillus subtilis CU1065. To understand cold shock effects, cells were cultivated at 37°C to log phase (O.D600=0.5-0.6) and then temperature was shifted to 15°C. Cold shock slow down the growth rate of wild type and deleted strains of DEAD-box RNA helicase gene (ydbR, yfmL, yqfR, deaD). The growth rate of ydbR deleted strain is 5 times severely reduced compared to that of wild type strain (CU1065). But the growth rate of other three (yfmL, yqfR, deaD) deleted strains is nearly equal to the growth rate of wild type. Compared to 37°C, the amount of ydbR and yqfR mRNA transcripts are increased at the growth temperature of 15°C. On the other hands the mRNA transcripts of yfmL and deaD are not changed at both conditions of 37°C and 15°C. Upon cold shock treatment ydbR mRNA transcript is clearly increased. After treatment of rifampicin (bacteria transcription inhibitor) the amount of ydbR mRNA was measured. Temperature shift from 37°C to 15°C and rifampicin treatment showed slowly decay of ydbR mRNA. But at 37°C and rifampicin treatment ydbR mRNA is rapidly reduced. These results showed that cold shock induction of ydbR mRNA resulted from the stability of ydbR mRNA and not from the transcription induction of ydbR. In relation to these results, we found the cold box element of csp (cold shock protein gene) in 5′ untranslated region of ydbR gene. Cold shock induction of ydbR is caused by the stability of ydbR mRNA like the stability of csp mRNA.
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KEYWORD
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Bacillus subtilis, cold shock, DEAD-box RNA helicase, rifampicin, RNA chaperone
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